Shc1^tm1Paw

2.0 kb of sequence, including exons 2 and 3, was replaced with a neomycin selection cassette. The deleted region contained the start codons for the three known isoforms and encoded portions of the PTB domain in each. Though transcript was undetected in homozygous mutant mice, a truncated protein (40 kD) was identified by Western blot analysis of total embryonic RNA. RT-PCR and in vitro transcription/translation analysis showed aberrant splicing involving the neo transgene and initiation of translation to be at codon 134. (J:62182)