Tg(EEF1A1-SHC1*)1Ravi

A construct was generated in which a human EF-1alpha promoter was separated from a modified human SHC1 cDNA fused to an IRES-GFP by a floxed stop codon. The floxed stop codon allowed transcriptional control via the expression of cre recombinase. SHC1 codons encoding three tyrosine residues (positions 239, 240, 317) that are phosphorylated after cross linking were modified to encode phenylalanine. This creates a dominant-negative protein in certain signaling pathways. Breeding of mice carrying this allele with transgenic mice expressing cre resulted in the expression of the modified SHC1, though not GFP. (J:78180)