Rorc^tm1Litt

A 200 bp fragment of the gene containing the exon encoding the DNA-binding domain was replaced with a neomycin resistance cassette via homologous recombination. Gene expression was undetectable in various tissues from homozygous mutant animals as determined by Northern blot analysis using a full-length cDNA probe. Western blot analysis did not detect protein expression in thymus of homozygous mutants. (J:63146)