在插入新霉素选择座后,它干扰了编码激酶域子区域I的外显子。通过 Western blot 分析,我们确证了纯合子小鼠中缺失了该蛋白。(来源:J:29796, J:97055)
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The insertion of a neomycin selection cassette disrupted the exon encoding the subdomain I of the kinase domain. The absence of protein in mice homozygous for the allele was verified by Western blot analysis. (J:29796, J:97055)