Hck^tm1Hev

This dicistronic locus was disrupted by the insertion of a neomycin selection cassette downstream of both start codons. Elongated transcript, containing the neo transgene, and a shortened transcript, resulting from the splicing of exons 1 and 3, were detected in homozygous mutant mice by Northern blot and RT-PCR analyses. Normal protein was undetected by Western blot analysis of bone marrow, and though the absence of truncated carboxy terminal peptide was not directly confirmed, assays of immunoprecipitates verified a lack of protein kinase activity. (J:16994)