外显子1及周边的基因组序列被替换成PGK-neo基因座。通过检测各组织提取物中的 Western blot 来确定纯合突变小鼠中编码蛋白的缺失。(来源:J:60597)
查看原文 参与反馈
Exon 1 and surrounding genomic sequence were replaced with a PGK-neo casstte. The absence of encoded protein in homozygous mutant mice was determined via Western blot analysis of extracts from various tissues. (J:60597)