外显子3被替换成新霉素抗性筛选 cassette。同源突变ES细胞的 Western blot 分析显示,既无正常蛋白也无剪切片段。(来源:J:50357)
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Exon 3 was replaced with a neomycin selection cassette. Western blot analysis of homozygous mutant ES cells showed an absence of both normal and truncated protein. (J:50357)