使用了一种撞击和逃跑类型的载体,在exon 1的第五个密码子位置产生了一个5个碱基的插入。对来自E13.5纯合小鼠的提取物进行的 Western blot 分析证实,这个等位基因不产生可检测到的蛋白质。(来源:J:20442)
查看原文 参与反馈
A hit and run type vector was used to create a 5 bp insertion in exon 1 at the fifth codon. Western blot analysis on extracts derived from E13.5 homozygous mice confirmed that no detectable protein was produced from this allele. (J:20442)