Exon 3被新霉素抗性筛选 cassette 的插入所打断。通过 Western blot 分析,我们确认了纯合突变小鼠中编码蛋白的缺失。(来源:J:78010)
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Exon 3 was disrupted by the insertion of a neomycin selection cassette. The absence of endcoded protein in homozygous mutant mice was confirmed via Western blot analysis. (J:78010)