在 neomycin 选择座中,1到4号外显子被替换。脾脏提取物和小鼠胚胎成纤维细胞的 Western blot 分析证实了纯合突变小鼠中无蛋白表达。(来源:J:74737)
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A neomycin selection cassette replaced exons 1 through 4. Western blot analysis of splenic lysates and mouse embyronic fibroblasts confirmed the absence of protein in homozygous mutant mice. (J:74737)