Thrb^tm2Few

The deletion of 3 base pairs (GRCm39:chr14:4439427_4439429delGTG, p.T337del) in exon 9, corresponding to a deletion that results in thyroid horomone resistance in humans, was introduced via site-directed mutagenesis along with a neomycin selection cassette inserted into intron 8. The mutation affects the ligand-binding domain in the encoded peptide, which is common to both isoforms produced from this locus. (J:77623)