Clcn3^tm1Tjj

Exon 3, encoding the first transmembrane domain, and surrounding intronic sequence was replaced with a neomycin selection cassette inserted by homologous recombination. The absence of transcript in homozygous mutant mice was determined by an RNAse protection assay of brain RNA. Western blot analysis, using a specific antibody directed against the amino terminal region, indicated a lack of encoded protein. (J:77635)