在exon 1的编码区域被替换了新霉素选择标记 cassette。 Western blot分析证实了纯合突变小鼠中目标位点编码的蛋白缺失。(来源:J:77652)
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The coding region of exon 1 was replaced with a neomycin selection cassette. Western blot analysis confirmed the absence of protein encoded by the targeted locus in homozygous mutant mice. (J:77652)