Tnfrsf11b^tm1Wss

A neomycin selection cassette into exon 2 replaced sequence encoded the first 93 residues of the mature protein. This targeting strategy eliminated the first two cysteine-rich domains, which are essential for function, and introduced an in-frame stop codon. Northern blot analysis of total hepatic RNA confirmed the absence of transcript in homozygous mutant mice. (J:47602)