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中文
为去除isoform A的翻译，同时保留isoform B，对isoform A特有的起始密码子166位的翻译位点进行了修改，编码为Alanine。为筛选，引入了无害突变，产生了一个新的NheI酶切位点，并插入了内含子2的floxed Neo标记选择子。重组的ES细胞通过转染表达Cre重组酶的质粒，实现了Neo选择子的剔除。对雌激素处理的纯合突变小鼠子宫提取物进行免疫印迹分析，显示isoform A缺失，isoform B蛋白水平保持不变。（来源：J:77661）
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
