这个基因通过同源重组,用lacZ-neo cassette替换了编码外显子,从而失活。通过在纯合突变动物各组织中进行 Western blot 分析,证实了基因表达的缺失(来源:J:72720)。
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The gene was disrupted by replacement of a coding exon with a lacZ-neo cassette via homologous recombination. Absence of gene expression was demonstrated by Western blot analysis of various tissues from homozygous mutant animals. (J:72720)