通过同源重组,该基因的整个编码区域被lacZ-neo cassette替换。通过免疫组化分析、 Western blot 和 RT-PCR,我们确认了纯合突变动物中基因表达的缺失。(来源:J:72798)
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The entire coding region of the gene was replaced with a lacZ-neo cassette via homologous recombination. Absence of gene expression in homozygous mutant animals was confirmed by immunohistochemical analysis, Western blot and RT-PCR. (J:72798)