Exon 1被替换为一个SA-lacZ-PGK-neo的同源重组 cassette,通过这种方式在各种来自纯合突变动物的组织中,通过 Western blot 分析检测不到蛋白质产品。(来源:J:70424)
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Exon 1 was replaced with an SA-lacZ-PGK-neo cassette via homologous recombination. Protein product was undetectable in various tissues from homozygous mutant animals as determined by Western blot analysis. (J:70424)