一段3.5kb的基因组片段,包括5'的编码区域,被替换了抗氨苄青霉素的耐药基因 cassette。通过对纯合小鼠提取物进行 Western blot 检测,结果显示这个等位基因没有产生可检测到的蛋白质。(来源:J:62547)
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A 3.5 kb genomic fragment containing the 5' coding region was replaced with a neomycin resistance cassette. Western blot analysis on extracts from homozygous mice confirmed that no detectable protein was made from this allele. (J:62547)