Hsf2^tm1Mmr

The gene is disrupted by insertion of a lacZ-neo cassette into exon 5 via homologous recombination. The gene targeting event results in the disruption of the oligomerization domain at lysine 167 and disruption of one of two nuclear localization signals while HSE-binding activity is retained. The mutant protein is expected to be cytoplasmic and lack trimerization abilities. RT-PCR and Western blot analyses confirmed the absence of gene expression in testes of homozygous mutant animals and homozygous mutant embryos. (J:76942)