6和7号外显子的大部分被诺姆酮选择标记取代,这个取代区域编码了编码蛋白的所有锌指。对纯合突变胚胎组织的 Western blot 检测显示没有蛋白质表达。(来源:J:63121)
查看原文 参与反馈
The majority of exons 6 and 7 was replaced with a neomycin selection cassette. The replaced region encoded all zinc fingers of the encoded protein. Western blot analysis of homozygous mutant embryonic tissue showed an absence of protein. (J:63121)