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中文
Exon 12，编码催化域的5和6个亚域，被替换了 neo 氨苄青霉素抗性筛选标记。从ES细胞提取的总RNA进行Northern blot分析，检测到了含有neo转基因的新转录本。RT-PCR结果显示，转录起源于内源性启动子，并延伸到了转基因中。 Western blot分析在纯化细胞裂解物中未检测到编码蛋白。通过免疫复合物激酶自磷酸化实验，确认了该蛋白功能的丧失。(来源：J:53258)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
