Pim1^tm1Pwl

A neomycin selection cassette replaced the promoter region, both transcriptional and translational start sites, and a portion of the coding sequence containing an essential lysine residue in the ATP-binding site of the protein kinase domain. An in vitro autophosphorylation assay showed an absence of phospho-kinase activity in splenocytes obtained from homozygous mutant mice. (J:15268)