Lat^tm1.1Mal

Tyrosine codon 136 in exon 7 was changed to a phenylalanine (p.Y136F) through an A-to-T mutation. In addition to the intended mutation, a new Bgl II restriction enzyme cleavage site was introduced in the intron 3' of exon 7 to accommodate a loxP site flanked neomycin resistance gene. Founder mice were bred to deleter mice to eliminate the floxed neo cassette leaving a single loxP site in intron 7. Western blot analysis of CD4-positive T cells verified the presence of protein product in mutant animals. (J:77098)