通过同源重组,将编码前P环的部分序列替换成PGK-neo cassette。纯合突变胚胎在E9.5阶段的整体 Western blot 分析中未显示出基因表达(来源:J:55332)
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Sequences encoding the anterior half of the P loop were replaced with a PGK-neo cassette via homologous recombination. Homozygous mutant embryos lacked gene expression as demonstrated by Western blot analysis of whole E9.5 embryos. (J:55332)