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An engineered point mutation in codon 719 resulted in a tyrosine to phenylalanine substitution (Y719F). Western blot analysis showed the level of mutant protein in homozygous mutant mice to be similar to that of the wild-type protein in wild-type mice. PI 3'-kinase activity was determined to be reduced by approximately 90% via assays of the PI 3'-kinase dependent activation of Akt. (J:60192)