该基因的第一个外显子通过同源重组被抗氨苄青霉素抗性基因座替换。通过对纯合突变动物肝脏提取物进行免疫沉淀和 Western 印迹,确认了该基因没有表达(来源:J:77412)
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The first exon of the gene was replaced with a neomycin resistance cassette via homologous recombination. Immunoprecipitation and Western blot of liver extracts from homozygous mutant animals verified the absence of gene expression. (J:77412)