Cenpb^tm2Khac

Stop codons were introduced into all three reading frames of the intronless gene by the insertion a translation frameshift linker into the amino-terminal centromere DNA-binding domain. An IRES-hygromycin selection marker was inserted in the 3' untranslated region upstream of the poly adenylation signal. Mice possessing both this allele and Cenpbtm1Khac were shown to lack Cenpb transcript and no CENPB protein was detected by immunofloureescence staining of metaphase chromosomes. (J:47171)