通过同源重组,将编码蛋白质催化区域的外显子替换为诺卡因抗性基因 cassette, Western blot 证实了纯合突变动物大脑中基因表达的缺失。(来源:J:75775)
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The exon encoding the catalytic domain of the protein was replaced with a neomycin resistance cassette via homologous recombination. Western blot analysis confirmed the absence of gene expression in brain of homozygous mutant animals. (J:75775)