6-8号外显子被替换成了一种诺卡因抗性基因座,通过同源重组实现。敲除等位基因通过 Western blot 分析在纯合突变动物大脑中检测到无蛋白产物来确认。(来源:J:77110)
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Exons 6-8 were replaced with a neomycin resistance cassette via homologous recombination. The knockout allele was confirmed via the absence of protein product in brain of homozygous mutant animals as determined by Western blot analysis. (J:77110)