Cd34^tm1Szk

The insertion of neomycin selection cassette deleted a 0.72-kb region including portions of exon 7 and exon 8 and encoding the entire transmembrane domain (amino acids 270 through 326). Staining of bone marrow cells isolated from homozygous mutant mice determined an absence of protein surface expression. Immunohistochemical staining and Western blot analysis confirmed a lack of protein in the lymph nodes of homozygous mutant mice. (J:32799)