Igdcc3^tm1Sms

The gene was disrupted by insertion of a PGK-neo-lacZ cassette into exon 2 via homologous recombination, which introduces stop codons into all 3 reading frames. LoxP sites flank the PGK-neo portion of the targeting vector. Northern blot analysis of E11.5 homozygous mutant embryos did not detect gene transcripts. RT-PCR detected very low amounts of mutant transcripts thought to originate within the lacZ gene. (J:70796)