Exon 5(编码102-130的氨基酸)通过同源重组被替换了neo抗药性基因。通过对纯合突变动物脾液的 Western blot 检测,确认了该基因没有表达。(来源:J:53513)
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Exon 5 (which encodes amino acids 102-130) was replaced with a neomycin resistance gene via homologous recombination. Western blot assay of spleen lysates from homozygous mutant animals verified the absence of gene expression. (J:53513)