在新霉素选择座插入的情况下,删除了1和2号外显子。通过 Western blot 分析,来自纯合突变胚胎的提取物中未检测到蛋白质。(来源:J:37092)
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The insertion of a neomycin selection cassette deleted exons 1 and 2. Protein was not detected in lysates from homozygous mutant embryos by Western blot analysis. (J:37092)