Adcyap1r1^tm1.1Gsc

ES cells were transfected with a construct in which a LoxP site was inserted just upstream of exon 11 and a floxed neomycin-thymidine-kinase region inserted downstream. The transient expression of cre recombinase in ES cells resulted in the excision of the selection genes and exon 11, which encodes a portion of the transmembrane domain IV and is included in all known splice variants. (J:76715)