将exons 2和3替换为无启动子的IRES-β-geo插件,通过同源重组。对纯合突变动物的肾脏进行 Western blot 分析,确认了基因表达的缺失。(来源:J:76209)
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Exons 2 and 3 were replaced with a promoter-less IRES-beta-geo cassette via homologous recombination. Western blot analysis of kidneys from homozygous mutant animals confirmed absence of gene expression. (J:76209)