Gaa^tm1.1Rabn

This allele is a derivative of Gaatm1Rabn in which Cre-mediated recombination in vivo excised the disrupted exon 6 and the neo gene under control of an adenovirus EIIa promoter, which confines the expression of Cre recombinase to an early stage of preimplantation development. RT-PCR analysis of muscle detected transcript produced by this allele of the size expected with exon 6 removed. Western blot analysis using rabbit antiserum to the human placental protein did not detect protein in liver from homozygous mutant mice. (J:48839)