在启动子编码区上游104bp位置插入了loxP引导的诺卡因抗性基因座,对纯合小鼠脑组织的 Western blot 分析显示,这个等位基因未检测到产生可检测到的蛋白质。(来源:J:72714)
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A loxP-flanked neomycin resistance cassette was inserted 104 bp upstream of the initiation codon. Western blot analysis on brain extracts derived from homozygous mice confirmed that no detectable protein is produced from this allele. (J:72714)