一种新霉素选择 cassette 替换了第一个编码外显子及周边序列。对来自纯合小鼠的脑组织匀浆进行 Western blot 分析后,确认未检测到任何可检测到的编码蛋白。(来源:J:59180)
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A neomycin selection cassette replaced the first coding exon and flanking sequences. Western blot analysis on brain lysates derived from homozygous mice confirmed that no detectable encoded protein was made. (J:59180)