通过同源重组,Exons 1和2被替换了 neo抗药性基因。在纯合突变动物的大脑中,通过 Western blot 分析,没有检测到蛋白质产品。(来源:J:76135)
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Exons 1 and 2 were replaced with a neomycin resistance gene via homologous recombination. Protein product was absent in brain of homozygous mutant animals as determined by Western blot analysis. (J:76135)