Olig1^tm1(cre)Rth

The majority of the coding region for this gene was replaced with a cre-neo cassette via homologous recombination so that the cre coding sequence is joined in-frame to the endogenous gene's coding region immediately following the translation initiation site. In situ hybridization of spinal cord from homozygous mutant embryos did not detect transcripts from the endogenous gene. In spinal cord from E10.5-E12.5 embryos heterozygous for this allele and for the conditional (Cre recombinase activated) lacZ reporter allele Gt(ROSA)26Sortm1Sor, Xgal staining co-localized with in situ hybridizing mRNAs for motor neuron and oligodendrocyte markers; antibodies to beta-galactosidase co-localized with those to an oligodendrocyte marker, but not to an astrocyte marker. (J:75868)