Itk^tm1Litt

Exon 4 and part of exon 3 of this gene was replaced with a PGK(G)neopA cassette via homologous recombination. A stop codon was engineered at position 78 to further ensure a null allele. PCR and Southern blot analysis confirmed the presence of the targeted mutation, and Western blot analysis determined no Itk protein was present in thymocytes of homozygous mutant animals. (J:31230)