这个基因的1327到1336位的氨基酸序列被替换为了一个PGK-neo-PolyA插件,通过同源重组,以基因转录的方向。neo插件5'端插入了一个终止密码子,导致目标基因的DNA聚合酶区域丢失。Southern blot结果显示,ES细胞中成功实现了基因靶向。(来源:J:74111)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入,基因内删除
--
1
1
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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