包含第三外显子的基因片段被诺瓦克林选择标记替换,用作筛选。对双靶向ES细胞衍生的提取物进行 Western blot 分析,结果显示这个等位基因没有检测到产生可检测的蛋白质。(来源:J:74972)
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A genomic fragment containing exon 3 was replaced with a neomycin selection cassette. Western blot analysis on lysates derived from doubly targeted ES cells confirmed that no detectable protein was produced from this allele. (J:74972)