在第二个外显子中插入了新霉素抗性基因座。对双靶向ES细胞进行的 Western blot 分析显示,这个等位基因未检测到表达的蛋白质。(来源:J:4556)
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A neomycin resistance cassette was inserted into the second exon. Western blot analysis on doubly targeted ES cells demonstrated that no detectable protein was expressed from this allele. (J:4556)