Tg(Bmi1)33Mvl

Insertion of a transgene consisting of a Bmi1 gene linked to a Pim1 promoter, the immunoglobulin enhancer, and a Moloney murine leukemia virus LTR causes overexpression of BMI1 in thymus, bone marrow, and spleen. The promoter and enhancer module gives high levels of transgene expression in lymphoid cells and is active early in development. Northern blot analysis detected a highly abundant transgenic transcript of 3kb in 11.5 dpc transgenic embryos. In situ RNA hybridization studies show that BMI1 is ubiquitously overexpressed in 12.5 dpc transgenic embryos. (J:24505)