在基因序列的第97位密码子位置插入了新霉素抗性基因 cassette,通过 Western blot 和免疫沉淀实验,确认了这个等位基因没有表达出可检测到的蛋白质库((J:64)
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A neomycin resistance cassette was inserted into the genomic sequence at a position corresponding to codon 97. Western blot and immunoprecipitation experiments confirmed that no detectable protein was expressed from this allele. (J:63425)