在exon 2的中间插入了一段新霉素抗性基因 cassette。通过对转基因小鼠肝脏的膜和核提取物进行 Western blot 分析,结果显示这个等位基因没有检测到可表达的蛋白质。(来源:J:43570)
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A neomycin resistance cassette was inserted into the middle of exon 2. Western blot analysis on membrane and nuclear extracts of liver from homozygous mice confirmed that no detectable protein was expressed from this allele. (J:43570)