在基因的第三外显子中插入了新霉素抗性基因座。对纯合小鼠脑组织的 Western blot 分析显示,这个等位基因没有检测到可检测到的蛋白表达。(来源:J:37576)
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A neomycin resistance cassette was inserted into exon 3 of the gene. Western blot analysis on brain extracts from homozygous mice confirmed that no detectable protein is expressed from this allele. (J:37576)