Ptpn11^tm1Paw

A neomycin resistance cassette replaced a genomic fragment containing exon 3, which contains sequences encoding the SH2-N motif. RT-PCR analysis on total RNA derived from heterozygous mice demonstrated that a transcript was made from this allele that spliced exon 2 to exon 4, resulting in a message that retained the proper reading frame. Western blot analysis on heterozygous specimins confirmed that a mutant protein is expressed from this allele that is consistent with the size expected. (J:43740)